Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® β-Arrestin 1 siRNA I (+) using β-Arrestin 2 (C16D9) Rabbit mAb #3857 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-Arrestin 2 (C16D9) Rabbit mAb confirms specificity of β-Arrestin 1 siRNA I, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.
Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® β-Arrestin 1 siRNA I (+) using β-Arrestin 1/2 (D24H9) XP ® Rabbit mAb #4674 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-Arrestin 1/2 (D24H9) Rabbit mAb confirms silencing of β-Arrestin expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of β-Arrestin 1 siRNA.